Loop-mediated isothermal amplification (LAMP) method for fast detection of Campylobacter spp in meat food products and environmental objects of a processing plant’s

نویسندگان

چکیده

There is constant necessity of developing the accurate and fast methods for detection foodborne pathogens. Microorganisms Campylobacter genus are one main causes diseases worldwide. Fast identification at all stages food life cycle an efficient strategy to control campylobacteriosis. This article authors evaluated a commercial loop-mediated isothermal amplification (LAMP) system with bioluminescence, called as 3M™ Molecular Detection Analysis (MDA), which was used find in products help certain standard method, referred reference method. The results this study showed that LAMP based method features high specificity minimum determinability (sensitivity). has been shown be reliable spp. scarce presence (10 CFU/25 g) meat, meat products, well carcass swabs production facilities’ environment. analysis required about 24–27 hours achieve result. However will facilitate Campylobacter, it provides much easier faster Cam pylobacter spp., including jejuni/Campylobacter coli, than microbiological methods. tool prevent spreading contamination products.

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ژورنال

عنوان ژورنال: Teoriâ i Praktika Pererabotki Mâsa

سال: 2022

ISSN: ['2414-441X', '2414-438X']

DOI: https://doi.org/10.21323/2414-438x-2022-7-2-125-130